Introduction to Biosciences Notes and Videos

Compiled Notes of Introduction to Bioscience (AS 104/AS 204) for Engineering students 

**Syllabus**

• learners can find a number of Questions and Answers in these notes 
• Students are requested to use these notes as supplementary study material only. Consult your Course instructor for further details or refer the text books. 

Text books:

1. “Concepts in Biology” by Elden D Enger, Frederick C Ross and David B Bailey: TataMcGraw Hill Education Private Limited, New Delhi
2. “Biology” Text Book for Class XII by National Council of Educational Research and Training, New Delhi

 Reference Material:

• http://www.mhhe.com/enger14e
• http://www.biotech.ca/EN/history.html
• http://statwww.berkely.eu/users/terry/classes/s2601998/week8b/week8b/nod9.html
• Amgen 2004 About Biotechnology. http://amgen.com/rnd/biotechnology.html

• for any further queries or suggestions.... comment below or mail me:  arvindarya@hotmail.com

Download link Unit I ....Click here

Download link Unit II ....Click here

Download link Unit III ....Click here // Supplementry Study material //
Download link Unit IV....Click here
Download link Unit V....Click here LINK 1, LINK 2

Click on the images below to watch the animations 

DNA Fingerprinting

Cell division Animation 1

Cell division Animation 2

watch the videos below on important topics 

Human Reproduction (Female Reproductive System)

Human Reproduction (Fertilization)

Human Reproduction (Oogenesis)

Human Reproduction (Menstrual Cycle)

HOW WE are born

GENE CLONING 

GENOME Sequencing

STEM CELLS

Genetically Modified Food

Gel Electrophoresis

Polymerase Chain Reaction 

Cell Division

Cell Structure and Function

Cell Organelles and Function

Glycolysis

Electron Transport System

DNA Replication

Trascription 

Translation (Protein Synthesis)

Tanscription and Translation

Practical manual of Genetic engineering

Practical manual of Genetic engineering/ Molecular Biology/ Recombinant DNA Technology

Download the practical manual

• This practical manual has some basic experiments for the beginners pursuing graduation in Biotechnology or Allied field. 
• Plz post your comments about any practical related doubts or clarification. or mail me at arvindarya@hotmail.com, catechize20@gmail.com

Have fun 

Transfer-Messenger RNA

Transfer-messenger RNA (abbreviated tmRNA, also known as 10Sa RNA and by its genetic name SsrA) is a bacterial RNA molecule with dual tRNA-like and messenger RNA-like properties. The tmRNA forms a ribonucleoprotein complex (tmRNP) together with Small Protein B (SmpB), Elongation Factor Tu (EF-Tu), and ribosomal protein S1. In trans-translation, tmRNA and its associated proteins bind to bacterial ribosomes which have stalled in the middle of protein biosynthesis, for example when reaching the end of a messenger RNA which has lost its stop codon. The tmRNA is remarkably versatile: it recycles the stalled ribosome, adds a proteolysis-inducing tag to the unfinished polypeptide, and facilitates the degradation of the aberrant messenger RNA. In the majority of bacteria these functions are carried out by standard one-piece tmRNAs. In other bacterial species, a permuted ssrA gene produces a two-piece tmRNA in which two separate RNA chains are joined by base-pairing.
E. coli contains a small RNA, encoded by the ssrA gene, is synthesized as a 457 nt precursor RNA that is processed by RNaseE to a mature 363 nt RNA. This RNA is also known as tmRNA or 10Sa RNA.
The ssrA RNA has a number of important properties:

• Its secondary and tertiary structure partially resembles that of tRNA
  
  
• It can be charged with alanine
  
  
• It can be used as an mRNA which codes for a 10 amino acid long oligopeptide: ANDENYALAA.
  

The mechanism of action of the ssrA RNA is shown in the following figure:
When a ribosome stalls, the ssrA RNA charged with alanine is brought to the A-site of the ribosome by the SsrB protein. Peptidyl transferase activity transfers the nascent polypeptide to the alanine attached to ssrA.
The mRNA template is also displaced by the ssrA RNA. Further protein synthesis now uses ssrA as a template and ten further amino acids (ANDENYALAA) are added to the C-terminal end of the polypeptide.
However, the final two amino acids that are added (AA) mark the new protein for proteolysis by the two proteases ClpAP and ClpXP.
Thus any proteins that are only partially synthesized by stalled ribosomes can be rapidly destroyed and turned over.

further reading...

The tmRNA system for translational surveillance and ribosome rescue

 Biochemistry3107 - Fall 1999

Transfer-messenger RNA From Wikipedia

CSIR NET JRF (LIFE SCIENCE) MCQ

BASED ON UNIT I

Time limit 30 min

Password protected =   Yes
Password = AIMnet2013  (mind the capital and small letters)

click here to start the quiz

Instructions: 

• This quiz has 20 MCQs based on Unit I of CSIR NET LIFE SCIENCE. 
• For each correct answer 4 marks will be awarded and for each incorrect answer 1 mark will be deducted. 
• You can check your results simultaneously. 
• This is a trial quiz so don't forget to write your comments in this blog. 
• coming quizzes will be having 50 MCQ.. 
• you will find more quizzes on the same units. ...

GUD LUCK...

ARVIND ARYA

Proposed Dates of CSIR NET Dec. 2013 Life Science MCQ.. ..

Also Find it on my webpage coming soon